Coding

Part:BBa_K4359008:Design

Designed by: Ira Zibbu   Group: iGEM22_IISER_TVM   (2022-10-07)


ClyA-V5-Affi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 925
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 925
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 925
    Illegal BglII site found at 81
    Illegal BglII site found at 1096
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 925
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was designed with an EcoN1 site upstream and BamH1 site downstream to enable ligation into the E.coli expression vector pGEX-4T1. Additional nucleotides flanking the restriction site (six before and six after) were included to ensure efficient digestions by restriction enzymes. The sequence on the registry only includes the CDS.


Source

References